How Cultured Hippocampal Neurons And Proteins And Rna...

Furthermore, we also found Kif5C O/E increases PSD95 and synaptophysin levels in dendrites of cultured hippocampal neurons, which further strengthen our hypothesis that Kif5C plays an important role in synapse formation. Our preliminary data have suggested that knockdown of Kif5C impairs the storage of contextual fear memories. Detailed characterization of the spine and branching phenotype and RNA cargos by electrophysiology and imaging are in progress. These studies will bring novel insights into the mechanisms of memory, synapse signaling and RNA transport. References 1. Willemsen MH, Ba W, Wissink-Lindhout WM, de Brouwer AP, Haas SA, Bienek M, Hu H, Vissers LE, van Bokhoven H, Kalscheuer V, Nadif Kasri N, Kleefstra T. Involvement of the kinesin family members KIF4A and KIF5C in intellectual disability and synaptic function. J Med Genet. 2014, 51(7): 487-94. 2. Liu XA, Kadakkuzha B, Pascal B, Steckler C, Akhmedov K, Yan L, Chalmers M, Puthanveettil SV. New approach to capture and characterize synaptic proteome. Proc Natl Acad Sci U S A. 2014,111(45): 16154-9. (B): The 2016 Cell Biology of the Neuron†¨Gordon Research Conference in Waterville Valley, NH is devoted to outstanding discoveries in neural development, function, regeneration as well as diseases. Neuronal cell biology has emerged as one of the most exciting and rapidly moving fields in contemporary biology. This field is experiencing a revolution in imaging technology and at the same time, powerful molecular andShow MoreRelatedLncrna Essay1706 Words   |  7 Pagesis heavily increased, without any significant change in the number of protein coding genes whereas non-coding portion of the genome has substantially undergone change. It is now well accepted fact that on increasing evolutionary levels, majority of the genome gives rise to non-coding RNAs (ncRNAs) that led to the increased biological complexity. By halting the central dogma – the flow of information from DNA to RNA to protein, ncRNAs have gained central attention. Recent advancement in the next generationRea d MoreIs The Amyloid Precursor Protein ( App ) Is Long Associated With Alzheimer s Disease?2728 Words   |  11 PagesIntroduction: The amyloid precursor protein (APP) is long associated with Alzheimer’s disease (AD). It is a single-pass transmembrane protein and is responsible for producing the neurotoxic AÃŽ ² plaque which accumulates within the brain (O’Brien et al. 2011). This accumulation of AÃŽ ² is what characterises AD. However, in spite of APP’s detrimental role in the pathogenesis of AD, it has been recently shown that APP can act as a neuroprotective molecule following traumatic brain injury (TBI). Approximately